In another aspect, the invention provides a method for identifying a protein interaction network comprising two or more bait proteins, comprising: (a) contacting said two or more bait proteins with a sample containing potential prey proteins, wherem the bait proteins and complexes comprising at least one said bait protein(s) are capable of being separated from other proteins in the sample; (b) separating said complexes comprising at least one said bait proteins and their prey proteins; (c) identifying prey proteins in the complexes using mass spectrometry, thereby identifying the protein interaction network.
In one embodiment, the protein interaction network comprises 5, 10, 20, 50, 100, 200 or more bait proteins.
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YEAST PROTEOME ANALYSIS Field of the Invention The invention relates to high-throughput proteome analysis. Background of the Invention Cellular behavior is determined by the dynamic interactions of a vast array of proteins that form complexes and higher order networks.
Related Applications This application claims priority to U. Provisional Applications, 60/323,930, filed on Sept. The global coordination of cellular function is presumed to require the concerted regulation of such networks.
Examples of the heterologous polypeptides include: GST, HA epitope, c-myc epitope, 6-His tag, FLAG tag, biotin, or MBP.